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The Use of Droplet Digital PCR to Quantify HIV-1 Replication in the Colorectal Explant Model

Ex vivo explant models are used to characterize in vitro efficacy of preexposure prophylaxis (PrEP) agents. Tissue is challenged with virus in culture and HIV-1 p24 levels are quantified with enzyme-linked immunosorbent assay (ELISA) on supernatants collected throughout a 14–21-day incubation. Due t...

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Dettagli Bibliografici
Pubblicato in:AIDS Res Hum Retroviruses
Autori principali: Myerski, Ashley, Siegel, Aaron, Engstrom, Jarret, McGowan, Ian, Brand, Rhonda M.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Mary Ann Liebert, Inc., publishers 2019
Soggetti:
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6434591/
https://ncbi.nlm.nih.gov/pubmed/30618283
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/aid.2018.0227
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