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The Use of Droplet Digital PCR to Quantify HIV-1 Replication in the Colorectal Explant Model

Ex vivo explant models are used to characterize in vitro efficacy of preexposure prophylaxis (PrEP) agents. Tissue is challenged with virus in culture and HIV-1 p24 levels are quantified with enzyme-linked immunosorbent assay (ELISA) on supernatants collected throughout a 14–21-day incubation. Due t...

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Detalhes bibliográficos
Publicado no:AIDS Res Hum Retroviruses
Main Authors: Myerski, Ashley, Siegel, Aaron, Engstrom, Jarret, McGowan, Ian, Brand, Rhonda M.
Formato: Artigo
Idioma:Inglês
Publicado em: Mary Ann Liebert, Inc., publishers 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6434591/
https://ncbi.nlm.nih.gov/pubmed/30618283
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/aid.2018.0227
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