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Isolation, culture and characterization of primary mouse RPE cells

Mouse models are powerful tools for the study of ocular diseases. alterations in the morphology and function of the retinal pigment epithelium (RPE) are common features shared by many ocular disorders. We report a detailed protocol to collect, seed, culture and characterize RPE cells from mice. We d...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Rosario, Fernandez-Godino, Garland, Donita L, Pierce, Eric A
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6432639/
https://ncbi.nlm.nih.gov/pubmed/27281648
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.065
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