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Isolation, culture and characterization of primary mouse RPE cells

Mouse models are powerful tools for the study of ocular diseases. alterations in the morphology and function of the retinal pigment epithelium (RPE) are common features shared by many ocular disorders. We report a detailed protocol to collect, seed, culture and characterize RPE cells from mice. We d...

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Bibliografiske detaljer
Udgivet i:Nat Protoc
Main Authors: Rosario, Fernandez-Godino, Garland, Donita L, Pierce, Eric A
Format: Artigo
Sprog:Inglês
Udgivet: 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6432639/
https://ncbi.nlm.nih.gov/pubmed/27281648
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.065
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