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Directed evolution studies of a thermophilic Type II-C Cas9

Though making up nearly half of the known CRISPR–Cas9 family of enzymes, the Type II-C CRISPR–Cas9 has been underexplored for their molecular mechanisms and potential in safe gene editing applications. In comparison with the more popular Type II-A CRISPR–Cas9, the Type II-C enzymes are generally sma...

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Detalles Bibliográficos
Publicado en:Methods Enzymol
Main Authors: Hand, Travis H., Das, Anuska, Li, Hong
Formato: Artigo
Idioma:Inglês
Publicado: 2018
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6423443/
https://ncbi.nlm.nih.gov/pubmed/30691646
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2018.10.029
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