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Expanding the scope of single and dual noncanonical amino acid mutagenesis in mammalian cells using orthogonal polyspecific leucyl-tRNA synthetases

Engineered aminoacyl-tRNA synthetase (aaRS)/tRNA pairs that enable site-specific incorporation of noncanonical amino acids (ncAAs) into proteins in living cells have emerged as powerful tools in chemical biology. The E. coli derived leucyl-tRNA synthetase (EcLeuRS)/tRNA pair is a promising candidate...

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Detalhes bibliográficos
Publicado no:Biochemistry
Main Authors: Zheng, Yunan, Mukherjee, Raja, Chin, Melissa A., Igo, Peter, Gilgenast, Martin J., Chatterjee, Abhishek
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6413323/
https://ncbi.nlm.nih.gov/pubmed/29106828
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.7b00952
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