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Expanding the scope of single and dual noncanonical amino acid mutagenesis in mammalian cells using orthogonal polyspecific leucyl-tRNA synthetases
Engineered aminoacyl-tRNA synthetase (aaRS)/tRNA pairs that enable site-specific incorporation of noncanonical amino acids (ncAAs) into proteins in living cells have emerged as powerful tools in chemical biology. The E. coli derived leucyl-tRNA synthetase (EcLeuRS)/tRNA pair is a promising candidate...
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| Veröffentlicht in: | Biochemistry |
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| Hauptverfasser: | , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2017
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6413323/ https://ncbi.nlm.nih.gov/pubmed/29106828 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.7b00952 |
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