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Impact of Polymerase Fidelity on Background Error Rates in Next-Generation Sequencing with Unique Molecular Identifiers/Barcodes

Liquid biopsy and detection of tumor-associated mutations in cell-free circulating DNA often requires the ability to identify single nucleotide variants at allele frequencies below 0.1%. Standard sequencing protocols cannot achieve this level of sensitivity due to background noise from DNA damage an...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Filges, Stefan, Yamada, Emiko, Ståhlberg, Anders, Godfrey, Tony E.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6401092/
https://ncbi.nlm.nih.gov/pubmed/30837525
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-39762-6
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