A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae

With rapid progress in DNA synthesis and sequencing, strain engineering starts to be the rate-limiting step in synthetic biology. Here, we report a gRNA-tRNA array for CRISPR-Cas9 (GTR-CRISPR) for multiplexed engineering of Saccharomyces cerevisiae. Using reported gRNAs shown to be effective, this s...

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Bibliografiske detaljer
Udgivet i:Nat Commun
Main Authors: Zhang, Yueping, Wang, Juan, Wang, Zibai, Zhang, Yiming, Shi, Shuobo, Nielsen, Jens, Liu, Zihe
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2019
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6400946/
https://ncbi.nlm.nih.gov/pubmed/30837474
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-09005-3
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