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Selective profiling of ribosomes associated with yeast Upf proteins
Ribosomes associated with nonsense-mediated decay factors Upf1, Upf2, or Upf3 were purified by immunoprecipitation, and enrichment and stoichiometry of Upf factors and ribosomal proteins were analyzed by western blot and mass spectrometry. Using a small RNA library preparation protocol that eliminat...
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| Publié dans: | Methods |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2018
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6387845/ https://ncbi.nlm.nih.gov/pubmed/30593864 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2018.12.008 |
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