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Selective profiling of ribosomes associated with yeast Upf proteins

Ribosomes associated with nonsense-mediated decay factors Upf1, Upf2, or Upf3 were purified by immunoprecipitation, and enrichment and stoichiometry of Upf factors and ribosomal proteins were analyzed by western blot and mass spectrometry. Using a small RNA library preparation protocol that eliminat...

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Détails bibliographiques
Publié dans:Methods
Auteurs principaux: GANESAN, ROBIN, LESZYK, JOHN, JACOBSON, ALLAN
Format: Artigo
Langue:Inglês
Publié: 2018
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6387845/
https://ncbi.nlm.nih.gov/pubmed/30593864
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2018.12.008
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