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ABC cloning: An efficient, simple, and rapid restriction/ligase-free method
DNA cloning remains the primary step before the further investigation of gene function. Restriction enzyme-based cloning methods are still widely used and numerous restriction-free cloning techniques are available as alternatives. Here we describe a PCR-based cloning method named ABC cloning. This m...
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| Publié dans: | MethodsX |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Elsevier
2019
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6384314/ https://ncbi.nlm.nih.gov/pubmed/30834197 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2019.02.007 |
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