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ABC cloning: An efficient, simple, and rapid restriction/ligase-free method
DNA cloning remains the primary step before the further investigation of gene function. Restriction enzyme-based cloning methods are still widely used and numerous restriction-free cloning techniques are available as alternatives. Here we describe a PCR-based cloning method named ABC cloning. This m...
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| Опубликовано в: : | MethodsX |
|---|---|
| Главные авторы: | , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Elsevier
2019
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6384314/ https://ncbi.nlm.nih.gov/pubmed/30834197 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2019.02.007 |
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