A Simple Cloning-free Method to Efficiently Induce Gene Expression Using CRISPR/Cas9

Gain-of-function studies often require the tedious cloning of transgene cDNA into vectors for overexpression beyond the physiological expression levels. The rapid development of CRISPR/Cas technology presents promising opportunities to address these issues. Here, we report a simple, cloning-free met...

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Veröffentlicht in:Mol Ther Nucleic Acids
Hauptverfasser: Fang, Lyujie, Hung, Sandy S.C., Yek, Jennifer, El Wazan, Layal, Nguyen, Tu, Khan, Shahnaz, Lim, Shiang Y., Hewitt, Alex W., Wong, Raymond C.B.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society of Gene & Cell Therapy 2018
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6307107/
https://ncbi.nlm.nih.gov/pubmed/30594894
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.omtn.2018.11.008
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