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GPN does not release lysosomal Ca(2+) but evokes Ca(2+) release from the ER by increasing the cytosolic pH independently of cathepsin C

The dipeptide glycyl-l-phenylalanine 2-naphthylamide (GPN) is widely used to perturb lysosomes because its cleavage by the lysosomal enzyme cathepsin C is proposed to rupture lysosomal membranes. We show that GPN evokes a sustained increase in lysosomal pH (pH(ly)), and transient increases in cytoso...

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Bibliografiske detaljer
Udgivet i:J Cell Sci
Main Authors: Atakpa, Peace, van Marrewijk, Laura M., Apta-Smith, Michael, Chakraborty, Sumita, Taylor, Colin W.
Format: Artigo
Sprog:Inglês
Udgivet: The Company of Biologists Ltd 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6382017/
https://ncbi.nlm.nih.gov/pubmed/30617110
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/jcs.223883
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