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In vivo characterisation of fluorescent proteins in budding yeast

Fluorescent proteins (FPs) are widely used in many organisms, but are commonly characterised in vitro. However, the in vitro properties may poorly reflect in vivo performance. Therefore, we characterised 27 FPs in vivo using Saccharomyces cerevisiae as model organism. We linked the FPs via a T2A pep...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Botman, Dennis, de Groot, Daan Hugo, Schmidt, Phillipp, Goedhart, Joachim, Teusink, Bas
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6381139/
https://ncbi.nlm.nih.gov/pubmed/30783202
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-38913-z
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