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An in vivo detection system for transient and low‐abundant protein interactions and their kinetics in budding yeast

Methylation tracking (M‐Track) is a protein‐proximity assay in Saccharomyces cerevisiae, allowing the detection of transient protein–protein interactions in living cells. The bait protein is fused to a histone lysine methyl transferase and the prey protein to a methylation acceptor peptide derived f...

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Pubblicato in:Yeast
Autori principali: Brezovich, Andrea, Schuschnig, Martina, Ammerer, Gustav, Kraft, Claudine
Natura: Artigo
Lingua:Inglês
Pubblicazione: John Wiley and Sons Inc. 2015
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4949564/
https://ncbi.nlm.nih.gov/pubmed/25582094
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/yea.3063
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