Evaluation and application of RNA-Seq by MinION

The current RNA-Seq method analyses fragments of mRNAs, from which it is occasionally difficult to reconstruct the entire transcript structure. Here, we performed and evaluated the recent procedure for full-length cDNA sequencing using the Nanopore sequencer MinION. We applied MinION RNA-Seq for var...

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Veröffentlicht in:DNA Res
Hauptverfasser: Seki, Masahide, Katsumata, Eri, Suzuki, Ayako, Sereewattanawoot, Sarun, Sakamoto, Yoshitaka, Mizushima-Sugano, Junko, Sugano, Sumio, Kohno, Takashi, Frith, Martin C, Tsuchihara, Katsuya, Suzuki, Yutaka
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2019
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6379022/
https://ncbi.nlm.nih.gov/pubmed/30462165
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/dnares/dsy038
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