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Cost-effective and robust genotyping using double-mismatch allele-specific quantitative PCR

For a wide range of diseases, SNPs in the genome are the underlying mechanism of dysfunction. Therefore, targeted detection of these variations is of high importance for early diagnosis and (familial) screenings. While allele-specific PCR has been around for many years, its adoption for SNP genotypi...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Sci Rep
Hauptverfasser: Lefever, Steve, Rihani, Ali, Van der Meulen, Joni, Pattyn, Filip, Van Maerken, Tom, Van Dorpe, Jo, Hellemans, Jan, Vandesompele, Jo
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2019
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6377641/
https://ncbi.nlm.nih.gov/pubmed/30770838
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-38581-z
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