High-throughput PCR assay design for targeted resequencing using primerXL

Abstract Background Although the sequencing landscape is rapidly evolving and sequencing costs are continuously decreasing, whole genome sequencing is still too expensive for use on a routine basis. Targeted resequencing of only the regions of interest decreases both costs and the complexity of the...

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Detalhes bibliográficos
Main Authors: Steve Lefever, Filip Pattyn, Bram De Wilde, Frauke Coppieters, Sarah De Keulenaer, Jan Hellemans, Jo Vandesompele
Formato: Artigo
Idioma:Inglês
Publicado em: BMC 2017-09-01
Colecção:BMC Bioinformatics
Assuntos:
Primer design
Targeted resequencing
Variant confirmation next generation sequencing
Sanger sequencing
PCR assay
Acesso em linha:http://link.springer.com/article/10.1186/s12859-017-1809-3
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