High-throughput PCR assay design for targeted resequencing using primerXL

Abstract Background Although the sequencing landscape is rapidly evolving and sequencing costs are continuously decreasing, whole genome sequencing is still too expensive for use on a routine basis. Targeted resequencing of only the regions of interest decreases both costs and the complexity of the...

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Bibliografski detalji
Glavni autori: Steve Lefever, Filip Pattyn, Bram De Wilde, Frauke Coppieters, Sarah De Keulenaer, Jan Hellemans, Jo Vandesompele
Format: Artigo
Jezik:Inglês
Izdano: BMC 2017-09-01
Serija:BMC Bioinformatics
Teme:
Primer design
Targeted resequencing
Variant confirmation next generation sequencing
Sanger sequencing
PCR assay
Online pristup:http://link.springer.com/article/10.1186/s12859-017-1809-3
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