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Linear-hairpin variable primer RT-qPCR for MicroRNA

Here, we present a highly specific, sensitive and cost-effective system to quantify microRNA (miRNA) expression based on two-step RT-qPCR with EvaGreen detection chemistry, called linear-hairpin variable primer RT-qPCR. It takes advantage of the novel designed variable primer, which is initially des...

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Detalhes bibliográficos
Publicado no:Chem Sci
Main Authors: Lan, Lin, Guo, Qiuping, Nie, Hemin, Zhou, Chang, Cai, Qingyun, Huang, Jin, Meng, Xiangxian
Formato: Artigo
Idioma:Inglês
Publicado em: Royal Society of Chemistry 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6375362/
https://ncbi.nlm.nih.gov/pubmed/30842860
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c8sc04621b
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