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Influence of RT-qPCR primer position on EGFR interference efficacy in lung cancer cells

BACKGROUND: Real-time quantitative RT-PCR (RT-qPCR) is a "gold" standard for measuring steady state mRNA levels in RNA interference assays. The knockdown of the epidermal growth factor receptor (EGFR) gene with eight individual EGFR small interfering RNAs (siRNAs) was estimated by RT-qPCR...

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Detalhes bibliográficos
Main Authors: Chen, Gang, Kronenberger, Peter, Teugels, Erik, De Grève, Jacques
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3047432/
https://ncbi.nlm.nih.gov/pubmed/21369532
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1480-9222-13-1
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