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Linear-hairpin variable primer RT-qPCR for MicroRNA

Here, we present a highly specific, sensitive and cost-effective system to quantify microRNA (miRNA) expression based on two-step RT-qPCR with EvaGreen detection chemistry, called linear-hairpin variable primer RT-qPCR. It takes advantage of the novel designed variable primer, which is initially des...

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Vydáno v:Chem Sci
Hlavní autoři: Lan, Lin, Guo, Qiuping, Nie, Hemin, Zhou, Chang, Cai, Qingyun, Huang, Jin, Meng, Xiangxian
Médium: Artigo
Jazyk:Inglês
Vydáno: Royal Society of Chemistry 2018
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6375362/
https://ncbi.nlm.nih.gov/pubmed/30842860
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c8sc04621b
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