Influence of RT-qPCR primer position on EGFR interference efficacy in lung cancer cells

BACKGROUND: Real-time quantitative RT-PCR (RT-qPCR) is a "gold" standard for measuring steady state mRNA levels in RNA interference assays. The knockdown of the epidermal growth factor receptor (EGFR) gene with eight individual EGFR small interfering RNAs (siRNAs) was estimated by RT-qPCR...

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Bibliographic Details
Main Authors: Chen, Gang, Kronenberger, Peter, Teugels, Erik, De Grève, Jacques
Format: Artigo
Language:Inglês
Published: BioMed Central 2010
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Research
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3047432/
https://ncbi.nlm.nih.gov/pubmed/21369532
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1480-9222-13-1
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