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High-Efficiency Lentiviral Transduction of Human CD34(+) Cells in High-Density Culture with Poloxamer and Prostaglandin E2
Hematopoietic stem cell (HSC) gene therapy is curative for various hereditary diseases; however, high-efficiency transduction in HSCs remains crucial to improve the prospects for hemoglobinopathies. We previously optimized lentiviral transduction in human CD34(+) cells with serum-free medium contain...
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| Опубликовано в: : | Mol Ther Methods Clin Dev |
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| Главные авторы: | , , , , , , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
American Society of Gene & Cell Therapy
2019
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6370599/ https://ncbi.nlm.nih.gov/pubmed/30788387 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.omtm.2019.01.005 |
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