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High-Efficiency Lentiviral Transduction of Human CD34(+) Cells in High-Density Culture with Poloxamer and Prostaglandin E2

Hematopoietic stem cell (HSC) gene therapy is curative for various hereditary diseases; however, high-efficiency transduction in HSCs remains crucial to improve the prospects for hemoglobinopathies. We previously optimized lentiviral transduction in human CD34(+) cells with serum-free medium contain...

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Detaylı Bibliyografya
Yayımlandı:Mol Ther Methods Clin Dev
Asıl Yazarlar: Uchida, Naoya, Nassehi, Tina, Drysdale, Claire M., Gamer, Jackson, Yapundich, Morgan, Demirci, Selami, Haro-Mora, Juan J., Leonard, Alexis, Hsieh, Matthew M., Tisdale, John F.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: American Society of Gene & Cell Therapy 2019
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC6370599/
https://ncbi.nlm.nih.gov/pubmed/30788387
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.omtm.2019.01.005
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