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Evaluation of Oral Cavity DNA Extraction Methods on Bacterial and Fungal Microbiota

The objective of this study was to evaluate the most effective method of DNA extraction of oral mouthwash samples for use in microbiome studies that utilize next generation sequencing (NGS). Eight enzymatic and mechanical DNA extraction methods were tested. Extracted DNA was amplified using barcoded...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Rosenbaum, Jennifer, Usyk, Mykhaylo, Chen, Zigui, Zolnik, Christine P., Jones, Heidi E., Waldron, Levi, Dowd, Jennifer B., Thorpe, Lorna E., Burk, Robert D.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6365504/
https://ncbi.nlm.nih.gov/pubmed/30728424
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-38049-6
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