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Combined bacterial and fungal intestinal microbiota analyses: Impact of storage conditions and DNA extraction protocols

BACKGROUND: The human intestinal microbiota contains a vast community of microorganisms increasingly studied using high-throughput DNA sequencing. Standardized protocols for storage and DNA extraction from fecal samples have been established mostly for bacterial microbiota analysis. Here, we investi...

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Bibliographische Detailangaben
Veröffentlicht in:PLoS One
Hauptverfasser: Angebault, Cécile, Ghozlane, Amine, Volant, Stevenn, Botterel, Françoise, d’Enfert, Christophe, Bougnoux, Marie-Elisabeth
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2018
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6075747/
https://ncbi.nlm.nih.gov/pubmed/30074988
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0201174
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