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An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated Protein

In this protocol, we describe a simple microscopy-based method to assess the interaction of a microtubule-associated protein (MAP) with microtubules. The interaction between MAP and microtubules is typically assessed by a co-sedimentation assay, which measures the amount of MAP that co-pellets with...

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Dettagli Bibliografici
Pubblicato in:Bio Protoc
Autori principali: Zhu, Yili, Tan, Weimin, Lee, Wei-Lih
Natura: Artigo
Lingua:Inglês
Pubblicazione: Bio-Protocol 2018
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6363367/
https://ncbi.nlm.nih.gov/pubmed/30733975
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3110
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