Miniaturisation of high-throughput plasmid DNA library preparation for next-generation sequencing using multifactorial optimisation

High-throughput preparation of plasmid DNA libraries for next-generation sequencing (NGS) is an important capability for molecular biology laboratories. In particular, it is an essential quality control (QC) check when large numbers of plasmid variants are being generated. Here, we describe the use...

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Vydáno v:Synth Syst Biotechnol
Hlavní autoři: Suckling, Lorna, McFarlane, Ciaran, Sawyer, Chelsea, Chambers, Stephen P., Kitney, Richard I., McClymont, David W., Freemont, Paul S.
Médium: Artigo
Jazyk:Inglês
Vydáno: KeAi Publishing 2019
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6352296/
https://ncbi.nlm.nih.gov/pubmed/30723818
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.synbio.2019.01.002
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