Miniaturisation of high-throughput plasmid DNA library preparation for next-generation sequencing using multifactorial optimisation

High-throughput preparation of plasmid DNA libraries for next-generation sequencing (NGS) is an important capability for molecular biology laboratories. In particular, it is an essential quality control (QC) check when large numbers of plasmid variants are being generated. Here, we describe the use...

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Detalhes bibliográficos
Publicado no:Synth Syst Biotechnol
Main Authors: Suckling, Lorna, McFarlane, Ciaran, Sawyer, Chelsea, Chambers, Stephen P., Kitney, Richard I., McClymont, David W., Freemont, Paul S.
Formato: Artigo
Idioma:Inglês
Publicado em: KeAi Publishing 2019
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6352296/
https://ncbi.nlm.nih.gov/pubmed/30723818
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.synbio.2019.01.002
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