Miniaturisation of high-throughput plasmid DNA library preparation for next-generation sequencing using multifactorial optimisation

High-throughput preparation of plasmid DNA libraries for next-generation sequencing (NGS) is an important capability for molecular biology laboratories. In particular, it is an essential quality control (QC) check when large numbers of plasmid variants are being generated. Here, we describe the use...

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Bibliografiska uppgifter
I publikationen:Synth Syst Biotechnol
Huvudupphovsmän: Suckling, Lorna, McFarlane, Ciaran, Sawyer, Chelsea, Chambers, Stephen P., Kitney, Richard I., McClymont, David W., Freemont, Paul S.
Materialtyp: Artigo
Språk:Inglês
Publicerad: KeAi Publishing 2019
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Article
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC6352296/
https://ncbi.nlm.nih.gov/pubmed/30723818
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.synbio.2019.01.002
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