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Expression and X-ray Structural Determination of the Nucleoprotein of Lassa Fever Virus
We describe methods to express the nucleoprotein (NP) of Lassa fever virus (LASV) in E. coli, to purify and crystallize it using the sitting-drop vapor diffusion method. The crystals were screened using Rigaku micro-007 X-ray generator and a dataset was collected at resolution of 2.36 Å. The crystal...
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| Publié dans: | Methods Mol Biol |
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| Auteurs principaux: | , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2018
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6351422/ https://ncbi.nlm.nih.gov/pubmed/28986833 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6981-4_12 |
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