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Expression and X-ray Structural Determination of the Nucleoprotein of Lassa Fever Virus
We describe methods to express the nucleoprotein (NP) of Lassa fever virus (LASV) in E. coli, to purify and crystallize it using the sitting-drop vapor diffusion method. The crystals were screened using Rigaku micro-007 X-ray generator and a dataset was collected at resolution of 2.36 Å. The crystal...
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| Publicado en: | Methods Mol Biol |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2018
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6351422/ https://ncbi.nlm.nih.gov/pubmed/28986833 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6981-4_12 |
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