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Expression and X-ray Structural Determination of the Nucleoprotein of Lassa Fever Virus

We describe methods to express the nucleoprotein (NP) of Lassa fever virus (LASV) in E. coli, to purify and crystallize it using the sitting-drop vapor diffusion method. The crystals were screened using Rigaku micro-007 X-ray generator and a dataset was collected at resolution of 2.36 Å. The crystal...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Qi, Xiaoxuan, Wang, Wenjian, Dong, Haohao, Liang, Yuying, Dong, Changjiang, Ly, Hinh
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6351422/
https://ncbi.nlm.nih.gov/pubmed/28986833
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6981-4_12
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