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Expression and X-ray Structural Determination of the Nucleoprotein of Lassa Fever Virus
We describe methods to express the nucleoprotein (NP) of Lassa fever virus (LASV) in E. coli, to purify and crystallize it using the sitting-drop vapor diffusion method. The crystals were screened using Rigaku micro-007 X-ray generator and a dataset was collected at resolution of 2.36 Å. The crystal...
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| Yayımlandı: | Methods Mol Biol |
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| Asıl Yazarlar: | , , , , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
2018
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6351422/ https://ncbi.nlm.nih.gov/pubmed/28986833 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6981-4_12 |
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