Protein refolding based on high hydrostatic pressure and alkaline pH: Application on a recombinant dengue virus NS1 protein

In this study we evaluated the association of high hydrostatic pressure (HHP) and alkaline pH as a minimally denaturing condition for the solubilization of inclusion bodies (IBs) generated by recombinant proteins expressed by Escherichia coli strains. The method was successfully applied to a recombi...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Chura-Chambi, Rosa Maria, da Silva, Cleide Mara Rosa, Pereira, Lennon Ramos, Bartolini, Paolo, Ferreira, Luis Carlos de Souza, Morganti, Ligia
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2019
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6347194/
https://ncbi.nlm.nih.gov/pubmed/30682103
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0211162
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