Association of high pressure and alkaline condition for solubilization of inclusion bodies and refolding of the NS1 protein from zika virus

BACKGROUND: Proteins in inclusion bodies (IBs) present native-like secondary structures. However, chaotropic agents at denaturing concentrations, which are widely used for IB solubilization and subsequent refolding, unfold these secondary structures. Removal of the chaotropes frequently causes reagg...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Publicado no:BMC Biotechnol
Principais autores: Rosa da Silva, Cleide Mara, Chura-Chambi, Rosa Maria, Ramos Pereira, Lennon, Cordeiro, Yraima, de Souza Ferreira, Luís Carlos, Morganti, Ligia
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2018
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6291932/
https://ncbi.nlm.nih.gov/pubmed/30541520
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-018-0486-2
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registros relacionados