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Evaluation of scFv protein recovery from E. coli by in vitro refolding and mild solubilization process
BACKGROUND: The production of therapeutically active single chain variable fragment (scFv) antibody is still challenging in E. coli due to the aggregation propensity of recombinant protein into inclusion bodies (IBs). However, recent advancement of biotechnology has shown substantial recovery of bio...
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| Publicado no: | Microb Cell Fact |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6330739/ https://ncbi.nlm.nih.gov/pubmed/30642336 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-019-1053-9 |
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