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Exploring the challenges of computational enzyme design by rebuilding the active site of a dehalogenase

Rational enzyme design presents a major challenge that has not been overcome by computational approaches. One of the key challenges is the difficulty in assessing the magnitude of the maximum possible catalytic activity. In an attempt to overcome this challenge, we introduce a strategy that takes an...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Jindal, Garima, Slanska, Katerina, Kolev, Veselin, Damborsky, Jiri, Prokop, Zbynek, Warshel, Arieh
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6329970/
https://ncbi.nlm.nih.gov/pubmed/30587585
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1804979115
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