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Efficient Homologous Recombination in Mice Using Long Single Stranded DNA and CRISPR Cas9 Nickase
The CRISPR/Cas9 nickase mutant is less prone to off-target double-strand (ds)DNA breaks than wild-type Cas9 because to produce dsDNA cleavage it requires two guide RNAs to target the nickase to nearby opposing strands. Like wild-type Cas9 lesions, these staggered lesions are repaired by either non-h...
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| Published in: | G3 (Bethesda) |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Genetics Society of America
2018
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6325892/ https://ncbi.nlm.nih.gov/pubmed/30504134 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.118.200758 |
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