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Transport Assays for Sterol-binding Proteins: Stopped-flow Fluorescence Methods for Investigating Intracellular Cholesterol Transport Mechanisms of NPC2 Protein

In this chapter we describe the use of stopped flow fluorescence spectroscopy to analyze the kinetic mechanisms of protein mediated cholesterol transfer to, from, and between model membranes. These assays allow for the detection of protein-membrane interactions that may occur during cholesterol tran...

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Bibliografische gegevens
Gepubliceerd in:Methods Mol Biol
Hoofdauteurs: McCauliff, Leslie A, Storch, Judith
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2017
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6311532/
https://ncbi.nlm.nih.gov/pubmed/28205170
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6875-6_9
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