Denaturation-enhanced droplet digital PCR for liquid biopsies

BACKGROUND. While interest in droplet-digital PCR technology (ddPCR) for circulating-DNA (cfDNA) analysis is burgeoning, the technology is compromised by sub-sampling errors and the few clinical targets that can be analyzed from limited input DNA. The paucity of starting material acts as a ‘glass ce...

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Pubblicato in:Clin Chem
Autori principali: Fitarelli-Kiehl, Mariana, Yu, Fangyan, Ashtaputre, Ravina, Leong, Ka Wai, Ladas, Ioannis, Supplee, Julianna, Paweletz, Cloud, Mitra, Devarati, Schoenfeld, Jonathan D., Parangi, Sareh, Makrigiorgos, G. Mike
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2018
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6310690/
https://ncbi.nlm.nih.gov/pubmed/30274976
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1373/clinchem.2018.293845
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