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Enhanced detection of microsatellite instability using pre-PCR elimination of wild-type DNA homo-polymers in tissue and liquid biopsies

Detection of microsatellite-instability in colonoscopy-obtained polyps, as well as in plasma-circulating DNA, is frequently confounded by sensitivity issues due to co-existing excessive amounts of wild-type DNA. While also an issue for point mutations, this is particularly problematic for microsatel...

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Bibliographische Detailangaben
Veröffentlicht in:Nucleic Acids Res
Hauptverfasser: Ladas, Ioannis, Yu, Fangyan, Leong, Ka Wai, Fitarelli-Kiehl, Mariana, Song, Chen, Ashtaputre, Ravina, Kulke, Matthew, Mamon, Harvey, Makrigiorgos, G Mike
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6158611/
https://ncbi.nlm.nih.gov/pubmed/29635638
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky251
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