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Measuring cis-regulatory energetics in living cells using allelic manifolds

Gene expression in all organisms is controlled by cooperative interactions between DNA-bound transcription factors (TFs), but quantitatively measuring TF-DNA and TF-TF interactions remains difficult. Here we introduce a strategy for precisely measuring the Gibbs free energy of such interactions in l...

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Veröffentlicht in:eLife
Hauptverfasser: Forcier, Talitha L, Ayaz, Andalus, Gill, Manraj S, Jones, Daniel, Phillips, Rob, Kinney, Justin B
Format: Artigo
Sprache:Inglês
Veröffentlicht: eLife Sciences Publications, Ltd 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6301791/
https://ncbi.nlm.nih.gov/pubmed/30570483
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.40618
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