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Quantitative imaging of cis-regulatory reporters in living embryos

A confocal laser scanning microscopy method has been developed for the quantitation of green fluorescent protein (GFP) as a reporter of gene activity in living three-dimensional structures such as sea urchin and starfish embryos. This method is between 2 and 50 times more accurate than conventional...

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Detalhes bibliográficos
Main Authors: Dmochowski, Ivan J., Dmochowski, Jane E., Oliveri, Paola, Davidson, Eric H., Fraser, Scott E.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC130556/
https://ncbi.nlm.nih.gov/pubmed/12237411
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.202483199
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