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Protein engineering strategies for improving the selective methylation of target CpG sites by a dCas9-directed cytosine methyltransferase in bacteria

Mammalian gene expression is a complex process regulated in part by CpG methylation. The ability to target methylation for de novo gene regulation could have therapeutic and research applications. We have previously developed a dCas9-MC/MN protein for targeting CpG methylation. dCas9-MC/MN is compos...

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Detaylı Bibliyografya
Yayımlandı:PLoS One
Asıl Yazarlar: Xiong, Tina, Rohm, Dahlia, Workman, Rachael E., Roundtree, Lauren, Novina, Carl D., Timp, Winston, Ostermeier, Marc
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Public Library of Science 2018
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC6298699/
https://ncbi.nlm.nih.gov/pubmed/30562388
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0209408
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