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Methods for Expression, Purification, and Characterization of PqqE, a Radical SAM Enzyme in the PQQ Biosynthetic Pathway

PqqE is the first enzyme in the biosynthetic pathway of the redox cofactor pyrroloquinoline quinone (PQQ), catalyzing the formation of a carbon–carbon bond in the precursor peptide PqqA. PqqE is a radical S-adenosyl-l-methionine (SAM) (RS) enzyme, a family of enzymes that use the reductive cleavage...

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Detalles Bibliográficos
Publicado en:Methods Enzymol
Autores principales: Zhu, Wen, Martins, Ana M., Klinman, Judith P.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2018
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6287933/
https://ncbi.nlm.nih.gov/pubmed/30097100
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2018.04.002
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