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Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions

8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target an...

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Detalhes bibliográficos
Publicado no:Molecules
Main Authors: Ito, Kenichiro, Liu, Hongshan, Komiyama, Makoto, Hayashi, Tetsuya, Xu, Yan
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2013
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6269988/
https://ncbi.nlm.nih.gov/pubmed/24141242
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/molecules181012909
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