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Highly efficient scarless knock-in of reporter genes into human and mouse pluripotent stem cells via transient antibiotic selection

Pluripotent stem cells (PSCs) edited with genetic reporters are useful tools for differentiation analysis and for isolation of specific cell populations for study. Reporter integration into the genome is now commonly achieved by targeted DNA nuclease-enhanced homology directed repair (HDR). However,...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:PLoS One
Main Authors: Sluch, Valentin M., Chamling, Xitiz, Wenger, Claire, Duan, Yukan, Rice, Dennis S., Zack, Donald J.
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2018
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6264506/
https://ncbi.nlm.nih.gov/pubmed/30496180
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0201683
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