Scarless Genome Editing of Human Pluripotent Stem Cells via Transient Puromycin Selection

Genome-edited human pluripotent stem cells (hPSCs) have broad applications in disease modeling, drug discovery, and regenerative medicine. We present and characterize a robust method for rapid, scarless introduction or correction of disease-associated variants in hPSCs using CRISPR/Cas9. Utilizing n...

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Detalhes bibliográficos
Publicado no:Stem Cell Reports
Main Authors: Steyer, Benjamin, Bu, Qian, Cory, Evan, Jiang, Keer, Duong, Stella, Sinha, Divya, Steltzer, Stephanie, Gamm, David, Chang, Qiang, Saha, Krishanu
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2018
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5830934/
https://ncbi.nlm.nih.gov/pubmed/29307579
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.stemcr.2017.12.004
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