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Effective Isotope Labeling of Proteins in a Mammalian Expression System

Isotope labeling of biologically interesting proteins is a prerequisite for structural and dynamics studies by NMR spectroscopy. Many of these proteins require mammalian cofactors, chaperons, or posttranslational modifications such as myristoylation, glypiation, disulfide bond formation, or N- or O-...

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書誌詳細
出版年:Methods Enzymol
主要な著者: Sastry, Mallika, Bewley, Carole A., Kwong, Peter D.
フォーマット: Artigo
言語:Inglês
出版事項: 2015
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC6260808/
https://ncbi.nlm.nih.gov/pubmed/26577736
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2015.09.021
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