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Effective Isotope Labeling of Proteins in a Mammalian Expression System
Isotope labeling of biologically interesting proteins is a prerequisite for structural and dynamics studies by NMR spectroscopy. Many of these proteins require mammalian cofactors, chaperons, or posttranslational modifications such as myristoylation, glypiation, disulfide bond formation, or N- or O-...
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| Publicado en: | Methods Enzymol |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2015
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6260808/ https://ncbi.nlm.nih.gov/pubmed/26577736 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2015.09.021 |
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