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Screening and Improvement of an Anti-VEGF DNA Aptamer

To obtain an aptamer with a high affinity for vascular endothelial growth factor (VEGF), we focused on the receptor-binding domain (RBD) of VEGF as a target epitope. Three rounds of screening gave Vap7, which bound to the VEGF isoforms VEGF(121) and VEGF(165) with K(D) values of 1.0 nM and 20 nM, re...

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Detalhes bibliográficos
Publicado no:Molecules
Main Authors: Nonaka, Yoshihiko, Sode, Koji, Ikebukuro, Kazunori
Formato: Artigo
Idioma:Inglês
Publicado em: Molecular Diversity Preservation International 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6256979/
https://ncbi.nlm.nih.gov/pubmed/20110884
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/molecules15010215
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