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A split luciferase-based probe for quantitative proximal determination of Gα(q) signalling in live cells

The earlier an activation of a G protein-dependent signalling cascade at a G protein-coupled receptor (GPCR) is probed, the less amplificatory effects contribute to the measured signal. This is especially useful in case of a precise quantification of agonist efficacies, and is of paramount importanc...

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Foilsithe in:Sci Rep
Main Authors: Littmann, Timo, Ozawa, Takeaki, Hoffmann, Carsten, Buschauer, Armin, Bernhardt, Günther
Formáid: Artigo
Teanga:Inglês
Foilsithe: Nature Publishing Group UK 2018
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6249299/
https://ncbi.nlm.nih.gov/pubmed/30464299
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-35615-w
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