CRISPR Guide RNA Cloning for Mammalian Systems

The outlined protocol describes streamlined methods for the efficient and cost-effective generation of Cas9-associated guide RNAs. Two alternative strategies for guide RNA (gRNA) cloning are outlined based on the usage of the Type IIS restriction enzyme BsmBI in combination with a set of compatible...

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Publicat a:J Vis Exp
Autors principals: Nageshwaran, Sathiji, Chavez, Alejandro, Cher Yeo, Nan, Guo, Xiaoge, Lance-Byrne, Alissa, Tung, Angela, Collins, James J., Church, George M.
Format: Artigo
Idioma:Inglês
Publicat: MyJove Corporation 2018
Matèries:
Genetics
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6235376/
https://ncbi.nlm.nih.gov/pubmed/30346403
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57998
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